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ISO-1 stimulates angiogenesis in vitro . (A) Representative images of tubular structure formation of endothelial cells seeding on Cultrex ® matrix. Cells were exposed to N (M199 medium supplemented with 5% FBS), S (0.5% DMSO), Nic (1x10 -8 M) or incremental concentrations of ISO-1 (1x10 -10 -10 -4 M). Scale bar, 100 µm. Arrows indicate the branching points of tubular structures. (B) Analysis of tubular-like structure formation, including the number of branches from principal segments and the total length of tubular structures. * P<0.05 vs. N. n=3. N, negative control; S, solvent control; Nic, nicotine.

Journal: Biomedical Reports

Article Title: Pro-angiogenic potential of the isoxazole derivative ISO-1 via α7-nAChRs activation in human endothelial cells

doi: 10.3892/br.2025.2057

Figure Lengend Snippet: ISO-1 stimulates angiogenesis in vitro . (A) Representative images of tubular structure formation of endothelial cells seeding on Cultrex ® matrix. Cells were exposed to N (M199 medium supplemented with 5% FBS), S (0.5% DMSO), Nic (1x10 -8 M) or incremental concentrations of ISO-1 (1x10 -10 -10 -4 M). Scale bar, 100 µm. Arrows indicate the branching points of tubular structures. (B) Analysis of tubular-like structure formation, including the number of branches from principal segments and the total length of tubular structures. * P<0.05 vs. N. n=3. N, negative control; S, solvent control; Nic, nicotine.

Article Snippet: Cultrex ® extracellular matrix (cat. no. 3533-010-02) was acquired from Trevigen, Inc. (BioTechne).

Techniques: In Vitro, Negative Control, Solvent, Control